![]() ![]() (2008) DIG Application Manual for Filter Hybridization, R.A.S. Mol Biotechnol 7:79–84Įisel D, Grunewald-Janho S, Hloch P et al. McCabe MS, Power JB, de Latt AMM et al (1997) Detection of single-copy genes in DNA from transgenic plants by nonradioactive southern blot analysis. An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. Yao Q, Cong L, Chang JL et al (2006) Low copy number gene transfer and stable expression in a commercial wheat cultivar via particle bombardment. This experiment introduces your students to Southern blotting as a tool for DNA Fingerprinting in a hypothetical paternity determination. Luo S, Peng J, Kunpeng L et al (2011) Contrasting evolutionary patterns of the Rp1 resistance gene family in different species of poaceae. Knox AK, Dhillon T, Cheng H et al (2010) CBF gene copy number variation at Frost Resistance-2 is associated with levels of freezing tolerance in temperate-climate cereals. On the other hand, it was possible that a single signal of Southern blot analysis would be caused by the insertion of a single copy of TDNA or the insertion of two TDNAs in a tandem inverted orientation (Ingham et al., 2001). Plant Cell Rep 31:167–177īartlett JG, Alves SC, Smedley M et al (2008) High-throughput Agrobacterium-mediated barley transformation. These results indicated that the TD line carries two copies of TDNA in the genome. HOME LIBRARY Glossary Southern blot A Southern blot is a laboratory method used to detect specific DNA molecules from among a many other DNA molecules. ![]() Nat Protoc 1:518–525Ĭasu RE, Selivanova A, Perroux JM (2012) High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR. A Southern blot is a laboratory method used to detect specific DNA molecules from among a many other DNA molecules. Molecular combing may have superior analytical validity compared to Southern blot for determining D4Z4 contraction size, detecting mosaicism, and resolving borderline and indeterminate Southern blot results. Southern EM (1975) Detection of specific sequences among DNA fragments separated by Gel-electrophoresis. FSHD is characterized by a slowly progressive asymmetric wasting of muscles of the face, shoulder and upper arms. ![]()
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